The H22 cell line is a mouse hepatocellular carcinoma (HCC) cell line commonly used in cancer research. The H22 cell line was originally derived from a chemically induced hepatoma in a Balb/c mouse. The H22 cell line is characterized by its rapid growth rate and aggressive behavior, making it a useful model for studying HCC progression and potential treatments.

The H22 cell line is often used in preclinical studies to evaluate the efficacy and toxicity of potential therapeutic agents for HCC, including chemotherapy, immunotherapy, and gene therapy. Researchers also use the H22 cell line to investigate the molecular mechanisms underlying HCC pathogenesis, including genetic and epigenetic alterations.

To maintain the H22 cell line in culture, suitable culture medium is required, and the cells need to be maintained at appropriate temperature and humidity conditions. Regular monitoring of cell confluence and viability is also essential.

Overall, the H22 cell line is a valuable tool for cancer research and has contributed significantly to our understanding of HCC biology and the development of potential treatments.